Keith Pardee Assistant professor Leslie Dan Faculty of Pharmacy University of Toronto Affiliations
Canadian Research Chair (tier II) in Synthetic Biology and Human Health
Visiting scholar, Wyss Institute, Harvard University
Medicine by Design Investigator
Our lab works in the field of synthetic biology, specifically we are pioneering in vitro devices to host cell-free synthetic gene networks for broad applications in sensing and human health. Through these efforts we are using the cell-free platform to lower the cost and technical barriers of diagnostics, and producing new biotechnologies that can be embedded into handheld portable devices. To do this we freeze-dry the enzymes of transcription and translation, along with genetically encoded tools, into porous materials such as paper. We have used this approach to create a sterile and abiotic platform for low cost diagnostics for Ebola and Zika viruses, and have also created a platform for making vaccines in the field. Through ongoing projects we are pushing the field in exciting directions and applying cell-free expression broadly to applications in biomedical research.